From the Science of Separations group at the “G. Ciamician " Department of Chemistry, Bologna, focused on separation techniques of nano- and micro-sized analytes, byFlow was born - a spinoff aimed at developing miniaturizated F4 technology. Subsequently, byFlow has evolved towards the development of technologies, methods and analyzes made available to third parties. The services offered include product characterization, applications in proteomics and pre-proteomics, stability and aggregation studies for pre-trial samples, morphology study (size / shape), and pre-marketing formulation studies of pharmaceuticals. Although FlFFF is at the core of byFlow's methodology, we offer numerous technical solutions.